(Department of Chemistry, 2015-07-30) Upadhyay, K.K
To further validate the feasibility of receptor 4 for the detection of Al3+ in vivo, confocal microscope images of HeLa cells incubated with receptor 4 were obtained. As shown in Figure 40, no fluorescence was observed in HeLa cells upon their incubation with 40 μg mL-1 of receptor 4 for 30 minutes. The cells were washed five times with PBS (Phosphate Buffered Saline) buffer solution and 250 μM Al3+ was added. The same is well within the range shown to elicit physiological responses (10-500 μM). After further incubation for 5 minutes, an enhancement of the fluorescence in the cells was observed.