Chitosan film of thiolated TPGS-modified Au-Ag nanoparticles for combating multidrug-resistant bacteria

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dc.contributor.authorSingh C.
dc.contributor.authorMehata A.K.
dc.contributor.authorViswanadh M.K.
dc.contributor.authorTiwari P.
dc.contributor.authorSaini R.
dc.contributor.authorSingh S.K.
dc.contributor.authorTilak R.
dc.contributor.authorTiwari K.N.
dc.contributor.authorMuthu M.S.
dc.date.accessioned2025-01-13T07:05:43Z
dc.date.available2025-01-13T07:05:43Z
dc.date.issued2024
dc.description.abstractThe widely used vitamin-E based amphiphilic material, tocopheryl polyethylene glycol succinate (TPGS) was further improvised to redox-sensitive thiolated TPGS (TPGS-SH), which has been achieved by attaching 4-aminothiophenol. Further, TPGS and TPGS-SH-coated bimetallic gold-silver nanoparticles (TPGS-Au-Ag-NP and TPGS-SH-Au-Ag-NP) were formulated to explore their antibacterial and wound healing abilities. The prepared NP were monodisperse with a mean hydrodynamic diameter of 69.5�3.23 nm, 59.65�3.23 nm, PDI of 0.3�0.05, 0.2�0.03 and a zeta potential of +29.2�2.71 mV and +35.28�1.53 mV, respectively. The TPGS and TPGS-SH modified Au-Ag-NP were tested for their antibacterial efficacy against methicillin-resistant Staphylococcus aureus (MRSA) and E. coli; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) results showed that TPGS Au-Ag-NP, TPGS-SH-Au-Ag-NP displayed significantly stronger antibacterial activity than their coating material alone. The anti-efflux pump activity of the most potent antibacterial agent TPGS-SH-Au-Ag-NP was evaluated against MRSA. Toxicity assessment of TPGS-SH-Au-Ag-NP in human dermal fibroblasts showed 88% viability up to 125 �g/mL. The TPGS-SH-Au-Ag-NP, after incorporation in chitosan film (TPGS-SH-Au-Ag-NP-CS), exhibited sustained release and prolonged in vivo residence characteristics, which were evaluated by IVIS. Results also revealed that treatment with TPGS-SH-Au-Ag-NP showed a significant 87% wound healing rate after 12 days of application in the rat model. Hence, we concluded that TPGS-SH-Au-Ag-NP was safe and effective against test bacteria MRSA and capable of efficient wound healing when incorporated in chitosan film. � 2024 Elsevier B.V.
dc.identifier.doi10.1016/j.colsurfa.2024.133287
dc.identifier.issn9277757
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/2020
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectAntibacterial
dc.subjectChitosan
dc.subjectEfflux pump
dc.subjectGold-silver bimetallic nanoparticles
dc.subjectThiolated TPGS
dc.subjectWound healing
dc.titleChitosan film of thiolated TPGS-modified Au-Ag nanoparticles for combating multidrug-resistant bacteria
dc.typeArticle
journal.titleColloids and Surfaces A: Physicochemical and Engineering Aspects
journalvolume.identifier.volume686

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