Potential effect of novel endophytic nitrogen fixing diverse species of Rahnella on growth promotion of wheat (Triticum aestivum L.)
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Date
2024
Journal Title
Journal of Crop Science and Biotechnology
Journal ISSN
Volume Title
Publisher
Springer
Abstract
The present investigation aims to isolate nitrogen fixing endophytic bacteria from cereals crops and their potential role in plant growth promotion of wheat (Triticum aestivum L.) for sustainable growth. In the present investigation, endophytic bacteria were isolated from different cereal crops growing in the Divine Valley of Baru Sahib, Himachal Pradesh, India and isolates were screened for nitrogen fixation. The nitrogenase activity exhibiting bacterial isolates were further screened for other plant growth promoting traits including solubilization of phosphorus, potassium, and zinc; production of indole-3-acetic acid, siderophores, ammonia, hydrogen cyanide and extracellular enzyme. The potential nitrogen fixing strains were molecularly identified and evaluated for the growth promotion of wheat. A total of 304 putative endophytic bacterial isolates were isolated from wheat, oats, barley, and maize using selective and complex growth media. Among 304 putative endophytic bacteria, 8 isolates exhibits nitrogenase activity. On the basis of nitrogenase activity and other plant promoting traits, two efficient strains i.e. EU-E1ST3.1 and EU-A2RNfb were molecularly identified using 16S rRNA gene sequencing and found that these strains belongs to genera Rahnella. The wheat inoculated with two selected nitrogen-fixing endophytic bacterial strains showed considerable enhancement in total chlorophyll, nitrogen, Fe and Zn content over the un-inoculated control. In comparison of two selected nitrogen-fixing endophytic bacterial strains, Rahnella aquatilis EU-E1ST3.1 was found to enhance better growth and physiological parameters and it might be developed as biofertilizers to establish a sustainable agriculture system. In the present investigation, the isolated potential nitrogen fixing endophytic bacteria could be used as biofertilizer or bioinoculant for growth of diverse cereal crops growing in hilly region for agricultural sustainability. � The Author(s), under exclusive licence to Korean Society of Crop Science (KSCS) 2024.
Description
Keywords
Endophytic bacteria, Physiological analysis, Plant growth promoting attributes, Rahnella, Wheat