Polyvinyl alcohol-chitosan based oleanolic acid nanofibers against bacterial infection: In vitro studies and in vivo evaluation by optical and laser Doppler imaging modalities

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dc.contributor.authorBadgujar P.
dc.contributor.authorMalik A.K.
dc.contributor.authorMehata A.K.
dc.contributor.authorSetia A.
dc.contributor.authorVerma N.
dc.contributor.authorRandhave N.
dc.contributor.authorShukla V.N.
dc.contributor.authorKande V.
dc.contributor.authorSingh P.
dc.contributor.authorTiwari P.
dc.contributor.authorMahto S.K.
dc.contributor.authorMuthu M.S.
dc.date.accessioned2025-01-13T07:03:34Z
dc.date.available2025-01-13T07:03:34Z
dc.date.issued2024
dc.description.abstractThe present work focuses on the fabrication of polyvinyl alcohol-chitosan-loaded oleanolic acid-nanofibers (PVA-CS-OLA-NFs) for bacterial infection. The prepared PVA-CS-OLA-NFs were characterized for contact angle, SEM, AFM, XRD, FTIR, and TGA. The solid-state characterization and in vitro performance evaluation of nanofibers reveal consistent interconnection and diameters ranging from 102 � 9.5 to 386 � 11.6 nm. The nanofibers have a flat surface topography and exhibit efficient drug entrapment. Moreover, the in vitro release profile of PVA-CS-OLA-NFs was found to be 51.82 � 1.49 % at 24 h. Furthermore, the hemocompatibility study showed that the developed PVA-CS-OLA-NFs are non-hemolytic to human blood. The PVA-CS-OLA-NFs demonstrate remarkable antibacterial capabilities, as evidenced by their MBC and MIC values, which range from 128 and 32 ?g/mL, against the strains of S. aureus. The in-vivo fluorescence optical imaging showed the sustained PVA-CS-OLA-NFs release at the wound site infected with S. aureus for a longer duration of time. Moreover, the PVA-CS-OLA-NFs showed superior wound healing performance against S. aureus infected wounds compared to the marketed formulation. Further, the laser Doppler imaging system improved oxygen saturation, blood supply, and wound healing by providing real-time blood flow and oxygen saturation information. � 2024 Elsevier B.V.
dc.identifier.doi10.1016/j.ijbiomac.2024.135532
dc.identifier.issn1418130
dc.identifier.urihttps://dl.bhu.ac.in/ir/handle/123456789/1183
dc.language.isoen
dc.publisherElsevier B.V.
dc.subjectBacterial infection
dc.subjectChitosan
dc.subjectElectrospinning
dc.subjectNanofibers
dc.subjectOleanolic acid
dc.subjectS. aureus
dc.subjectTopical delivery
dc.titlePolyvinyl alcohol-chitosan based oleanolic acid nanofibers against bacterial infection: In vitro studies and in vivo evaluation by optical and laser Doppler imaging modalities
dc.typeArticle
journal.titleInternational Journal of Biological Macromolecules
journalvolume.identifier.volume279

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